Abstract Evaluation of volatile aroma compounds from Chinese wild grape species aimed to explore new grape species via fruit aroma. In this study, the volatile organic compounds (VOCs) from ten grape cultivars belonging to nine different grape species were detected, by using headspace solid-phase microextraction with GC-MS. Chinese wild grape species were studied along with table grape (Muscat Hamburg, Concord) and wine grape (Cabernet Sauvignon) cultivars belonging to different origins. Total forty-five volatile compounds were detected, upon which several VOCs including pentanoic acid, cyclobutyl ester, maltol, (+)-neoisomenthol, and 8-methyloctahydrocoumarin were detected in Chinese wild grapes that have not been detected in other Vitis species. The results revealed that C6 volatile compounds were pre-dominant VOCs. Esters were found abundant in V. labrusca (Concord) and terpenoids were dominant in V. vinafera (Hamburg Muscat). The principal component analysis showed that Chinese wild grape cultivars V. yeshanensis, V. facifolia, V. davidii, V. betulifolia V. heyneana, V. amurensis and V. adenoclada are correlated with wine grape cultivar ‘Cabernet Sauvignon’, while table grape cultivars are distributed separately. These results can provide a reference for the utilization of new germplasm for specific purposes.

MicroRNAs (miRNAs) have been indicated to be frequently dysregulated in various cancers and promising biomarkers for colon cancer. The present study aimed to assess the prognostic significance and biological function of miR-1273a in colon cancer. The expression levels of miR-1273a was estimated using quantitative real-time polymerase chain reaction. Kaplan-Meier survival curves and Cox regression analysis were used to evaluate the prognostic value of miR-1273a in patients of colon cancer. The effects of miR-1273a on cell proliferation, migration, and invasion were investigated by cell experiments. The expression of miR-1273a was downregulated in colon cancer tissues and tumor cell lines compared with the normal controls (all P<0.001). The aberrant expression of miR-1273a was associated with vascular invasion (P=0.005), differentiation (P=0.023), lymph node metastasis (P=0.021), and TNM stage (P=0.004). The patients with low miR-1273a expression had low overall survival compared with the patients with high miR-1273a expression (log-rank P=0.002). miR-1273a was detected to be an independent prognostic biomarker for patients. Furthermore, the results of cell experiments revealed that miR-1273a downregulation promoted, while miR-1273a upregulation suppressed the cell proliferation, migration, and invasion. In conclusion, all data indicated that a downregulated expression of miR-1273a predicted poor prognosis for colon cancer and enhanced tumor cell proliferation, migration, and invasion. Thus, we suggest that methods to promote miR-1273a expression may serve as novel therapeutic strategies in colon cancer.

Abstract: To investigate the mechanism of different exercise patterns on neurological function after focal cerebral ischaemia in rats. Rats with focal cerebral cerebral ischaemia were randomly divided into an aerobic exercise group, an exhaustive exercise group and a control group, with 8 rats in each group. A score for nerve function in each group was calculated, and the ultrastructure of nerve cells was observed. Levels of NO and NOS in the brain motor area of the rats were measured in each group. The aerobic exercise group had lower nerve function scores than the exhaustive exercise group and higher scores than the control group (P<0.05). Under transmission electron microscopy, irregular shapes and organs were observed in nerve cells in the control group, while regular cell shapes and organs were observed in the aerobic exercise group. The aerobic exercise group and exhaustive exercise group had higher measures of NO content, NOS activity and eNOS, nNOS and iNOS gene expression than the control group, but eNOS expression in the aerobic exercise group and iNOS expression in the exhaustive exercise group were clearly higher according to RT-PCR (P<0.05). Aerobic exercise can promote the expression of NOS, mainly in eNOS, which can promote nerve repair.

Neste trabalho, propõe-se um novo método de remoção de ruído para imagens hiperspectrais avaliado com dados reais e simulados. Um cubo de predições de imagens hiperespectrais é calculado por regressão linear múltipla no domínio espectral com base na alta correlação do sinal útil e na não correlação do ruído aleatório nas bandas hiperespectrais É aplicada uma transformada complexa de wavelet bi-dimensional no domínio espectral derivado, no qual os níveis de ruído são temporariamente elevados para impedir a deformação do sinal durante a remoção do ruído, e então a compressão bivariada foi usado para compactar os coeficientes da transformada wavelet. Os resultados experimentais demonstraram que o método proposto possibilita obter resultados melhores do que outros métodos de remoção de ruídos encontrados na literatura, com melhoria da razão de ruído do sinal de 0.5dB para 10dB. Os dados reais utilizados mostraram que o método proposto é válido e efetivo.

In this paper, a new denoising method is proposed for hyperspectral remote sensing images, and tested on both the simulated and the real-life datacubes. Predicted datacube of the hyperspectral images is calculated by multiple linear regression in the spectral domain based on the strong spectral correlation of the useful signal and the inter-band uncorrelation of the random noise terms in hyperspectral images. A two dimensional dual-tree complex wavelet transform is performed in the spectral derivative domain, where the noise level is elevated temporarily to avoid signal deformation during the wavelet denoising, and then the bivariate shrinkage is used to shrink the wavelet coefficients. Simulated experimental results demonstrate that the proposed method obtains better results than the other denoising methods proposed in the reference, improves the signal to noise ratio up to 0.5dB to 10dB. The real-life data experiment shows that the proposed method is valid and effective.

Carbon sequestration in grassland soil has been paid considerable attention in recent decades. However, the changes of soil organic carbon (SOC) still need clarification under the effect of environmental factors in semi-arid area. Here, twenty sampling sites were selected to study the effects of plant community (plant cover, biomass, litter, composition and diversity) and environmental factors (i.e. mean annual precipitation, mean annual temperature) on SOC sequestration in the semi-arid grasslands. The results showed that SOC was significant positively related to mean annual precipitation, soil water content and soil pH. The higher above- and below-ground biomass, species evenness and diversity presented the higher SOC. Specially, the species richness and proportion of gramineous species functional group significantly increased SOC. Below-ground biomass affected SOC mainly in the top 30 cm soil. Our results suggest that higher plant species richness and gramineous species proportion play a positive role in increasing the potential of soil carbon sequestration in semi-arid grassland.

ZnO particles were prepared from Zn nanoparticles by thermal oxidation in air, over a temperature range of 500 to 900 ºC. The microstructure of ZnO was investigated by SEM and XRD, showing that the thermal oxidation temperature affected the particle growth mechanism, morphology, and microstructure. Positron annihilation spectroscopy indicated that the thermal oxidation process has an important influence on the zinc-related vacancies and their clusters in the ZnO particles.

For immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 on DEAE-52 cellulose, the optimal amount of enzyme protein was 12 mg protein: 1 g DEAE-52 cellulose; the optimal pH was 6.5; and the optimal immobilization time was 6 hr. The specific activity of immobilized enzyme was 36.67 mU.g-1 carrier with an immobilization yield of 98.87% and an enzyme recovery yield of 92.43%. The molar transformation rates of puerarin by immobilized enzyme and by the relative bacterial cell amount equal to the same amount of enzyme were 53.3% and 2.2%, respectively, after 1 hr of transformation. The former molar transformation rate, which was similar to that for free enzyme, was more than 24-fold greater than the latter. The immobilized puerarin glycosidase showed improved enzymatic properties and stability. The immobilized puerarin glycosidase retained 88% of its initial activity after being reused 10 times.

Estimativa do ruído de imagens hiperespectrais de sensoriamento remote é importante para o pós-processamento e aplicações. Nesta pesquisa, não somente a remoção da correlação espectral é considerada, como também a remoção da correlação espacial pela transformada wavelet . Portanto, um novo método baseado na regressão linear múltipla (MLR) com a transformada wavelet é proposto para estimar o ruído da imagem hiperespectrais de sensoriamento remoto. Uma simulação numérica de dados AVIRIS é executada, e dados reais Hyperion são utilizados para validar o algoritmo proposto. Resultados experimentais mostram que o método é mais adaptável e preciso do que o MLR geral e outros métodos classificados.

Noise estimation of hyperspectral remote sensing image is In this paper, not only the spectral correlation removing is considered, but the spatial correlation removing by wavelet transform is considered as well. Therefore, a new method based on multiple linear regression (MLR) and wavelet transform is proposed to estimate the noise of hyperspectral remote sensing image. Numerical simulation of AVIRIS data is carried out and the real data Hyperion is also used to validate the proposed algorithm. Experimental results show that the method is more adaptive and accurate than the general MLR and the other classified methods.